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  • 產(chǎn)品名稱(chēng)：IL-13 ELISA Kit(Interleukin13)

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IL-13 ELISA Kit(Interleukin13)
詳情介紹：
Purpose This immunoassay kit allows for the in vitro quantitative determination of human Interleukin 13, IL-13 concentrations in cell culture supernates, serum, plasma and other biological fluids.
Sample Type Cell Culture Supernatant, Serum, Plasma, Biological Fluids
Analytical Method Quantitative
Detection Method Colorimetric
Specificity This assay recognizes recombinant and natural human IL-13.
Cross-Reactivity (Details) No significant cross-reactivity or interference was observed.
Sensitivity The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
Characteristics Homo sapiens,Human,Interleukin-13,IL-13,IL13,NC30
Alternative Name IL13 (IL13 ELISA Kit Abstract)
Background Interleukin 13 (IL-13) is produced primarily by activated T lymphocytes. It shows approximately 30% amino acid (aa) sequence identity with IL-4 and shares many biological properties with IL-4. IL-13 has multiple effects on the differentiation and functions of monocytes/macrophages. It can suppress the cytotoxic functions of monocytes/macrophages and the production of proinflammatory cytokines by monocytes/macrophages. Although rat or mouse IL-13 has effects similar to those of IL-4 on rat B cells, mouse IL-13 has no effect on mouse B cells. Unlike IL-4, which is a growth and differentiation factor for rat and mouse T cells, IL-13 has no effects on either rat or mouse T cells. The biological effects of IL-13 are mediated by specific high-affinity cell surface receptor complexes. The functional IL-13 receptor complex has been shown to consist of the low-affinity IL-13 receptor α chain and the IL-4 receptor α subunit (IL-4 Rα). This IL-13 receptor complex may also serve as an alternate high-affinity IL-4 receptor complex in IL-4 responsive cells that lack the γ chain.
Pathways JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport
Sample Volume 100 μL
Plate Pre-coated
Protocol The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-13. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-13 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IL-13, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-13 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 2
Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.