產(chǎn)品詳情
簡單介紹:
Rabbitanti-Dog(Canine)IgG(WholeMolecule)Antibody(FITC)
詳情介紹:
Immunogen | Dog IgG whole molecule |
Characteristics | Molar Ratio: 2.4 moles DTAF per mole of Rabbit IgG |
Purification |
Immunoaffinity chromatography This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
Background | Synonyms: dog IgG, dog immunoglobulin G |
Research Area | Immunology, Secondary Antibodies |
Application Notes |
Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Comment |
Absorption / Emission: 495 nm / 528 nm |
Restrictions | For Research Use only |
Reconstitution | Restore with 1.0?mL of deionized water (or equivalent). |
Concentration | 2.0 mg/mL |
Buffer | 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride,?pH 7.4 with 10?mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01?% (w/v) Thimerisol as preservative |
Preservative | Thimerosal (Merthiolate) |
Precaution of Use | Biohazard Informations: This product contains thimerosal which is hazardous. |
Handling Advice |
Product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. |
Storage | 4 °C/-20 °C |
Storage Comment | Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid. |
Background publications |
Blakeslee, Baines: "Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG." in: Journal of immunological methods, Vol. 13, Issue 3-4, pp. 305-20, 1977 (PubMed).
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